Week 3 assignment:
Define aseptic transfer or aseptic technique.
2. Explain how to decontaminate your work area. What is the purpose of decontaminating your work area?
3. Inoculating loops are used for________________________________________.
4. Inoculating needles are used for______________________________________.
5. Explain how bacteria multiply.
6. Before an inoculating instrument can be used it must be_______________________________________.
7. Explain the procedure to light a Bunsen burner.
8. Name the instrument used to light the Bunsen burner.
9. Describe the procedure used in sterilizing the inoculating needle.
10. Why should the inoculating needle be allowed to cool? How long?
11. What does this mean “aerosol will be created?”
12. Explain the procedure for transferring or inoculating broth into a sterile tube.
13. To control spilling, how should the test tube be held?
14. When or how often should the test tube be sterilized?
15. Explain the stab procedure.
16. Why should you decontaminate your workstation after completing the lab?