This is a written assessment. No dot points are permitted. You may include section subheadings. 1500 words is the maximum word count, which excludes references and figure legends. There is no minimum word count. Referencing style is Vancouver
The content of the laboratory classes is directly linked with weekly content covered in Weeks 4-7. In the laboratory classes you will begin by investigating the fundamentals of flow cytometry and how the parameters of forward- and side-scatter (FSC and SSC) can be used to identify different cell populations. Using flow cytometry you will investigate the expression of activation-dependent cell surface markers following stimulation of the Jurkat T-cell acute lymphoblastic leukemia cell line using agonistic anti-CD3 and anti-CD28 antibodies. The report will have four sections:
1. A brief introduction that describes T-cell activation and the use of agonistic antibodies.
2. results section in which you are expected to correctly and accurately present and analyse the data generated in the laboratory classes. This includes: correct formatting of figures, including correctly labelled axis, and a f igure legend that describes what is being shown; a text that accompanies each figure, but is separate from the f igure legend, and that identifies key outcomes in each figure; correct statistical analysis of the figure (we will be using non-parametric tests to analyse the data)
3. the discussion, you will be required to provide a critical appraisal of your results and, using no more than five references, comment on the dynamic changes in gene expression and DNA methylation in the context of T-cell activation, and how genetic variation might impact on T-cell activation in the context of immune disease
4. reference list, correctly formatted with in-text citations using the Vancouver referencing style
PART 1. A brief introduction that describes antigen-dependent T-cell activation and compare this to T-cell activation using agonistic antibodies. (10marks)
PART 2. Flow cytometry figures (histograms and dot plots) are correctly presented and analysed (5 marks)
PART 2. qPCR figures showing and analysis of gene expression and an analysis of DNA methylation (5 marks)
PART 2. Statistical analysis has been correctly applied (additional information will be provided on vUWS and in tutorials on the use, type, formatting, and interpretation of the statistical test to be used) (5 marks)
PART 3. A critical appraisal of your results, integrating information from the experiments to reach a conclusion, and a consideration of how genetic variation might impact on T-cell activation in the context of immune disease (10 marks)
PART 4. Referencing (5 marks)
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Additionally, I have attached all the information required to complete this task. All results, practical notes and tutorial videos will be uploaded. Read through the practical slides carefully. vancouver referencing.
Watch this video for help –>
https://www.youtube.com/watch?v=U_eQ8VetQxw
Please aim for a distinction. I am happy to tip you.
