Please Note: For this assignment Turnitin Integrity software will be used. Please make sure that the submitted assignment is in your own words and NOT copied from other sources.
Please watch the following video about Enzyme linked Immunosorbent Assay (ELISA).
You can also refer to Chapter 18 of our textbook for more details.
There are two basic methods. The direct ELISA and the indirect ELISA.
For this assignment please compare and contrast the above two methods in detail. Explain the process of performing these tests in a step by step manner. Please Limit Your Post To 150 Words.
In Direct ELISA:
What is known (antigen or antibody)? What is the unknown? What is/are used as indicator (s)? What indicates a positive result? What are the application of Direct Elisa? Please provide at least two examples.
Please discuss the Indirect Elisa in Great detail as well. What is known (antigen or antibody)? What is the unknown? What is/are used as indicator (s)? What indicates a positive result? What are the application of Indirect Elisa? Please provide at least two examples.
INTRODUCTION: The science of Serology:
Microorganism are antigenic, which means they carry unusual molecules on their body. Examples are;
Teichoic acid in Gram-positive organisms
O polysaccharides in Gram-negative organisms
Flagellin proteins, which are the building blocks of flagella
Peptidoglycan in cell wall