Lab Report Directions
Write a lab report that includes the following:
A. Identify which unknown organism you will be researching and the attached “Lab Manual” you will use. Include these numbers in your lab report. Identify your unknown organism by doing the following:
• Find the group in the following table that correlates to the first initial of your last or family name.
• Select one of the four possible unknown organisms from the column within your group.
Note: For example, if your last name begins with H, you may choose from Unknown 5, 6, 7, or 8 in the table. If you chose Unknown 8, using the corresponding number, you would open the attached “Task 2 Unknown Lab Manual #8.”
Group 1 (A-F) Group 2 (G-L) Group 3 (M-R) Group 4 (S-Z)
Unknown 1 Unknown 5 Unknown 9 Unknown 13
Unknown 2 Unknown 6 Unknown 10 Unknown 14
Unknown 3 Unknown 7 Unknown 11 Unknown 15
Unknown 4 Unknown 8 Unknown 12 Unknown 16
Note: Use the “Decision Tree” located in Section 4 Lesson 2.7 as a guide while working through your chosen lab manual to decide which tests to explore to identify your unknown organism.
First Identification Test: Gram Stain
B. Describe the color and morphology of the cells observed in the Gram stain image provided in the lab manual and whether the unknown organism is gram-positive or gram-negative in your lab report.
Second Identification Test
C. Identify the second identification test to perform to learn about your unknown organism and explain why you chose this test.
1. Describe the basic steps needed to perform the second identification test.
2. Describe the observed results of the second identification test (e.g., color, presence of bubbles) and determine whether the test results are positive or negative.
3. Explain what the results of the second identification test tell you about the traits of the unknown organism chosen in part A.
Third Identification Test
D. Identify the third identification test to be performed and explain why you chose this test.
1. Describe the basic steps needed to perform the third identification test.
2. Describe the observed results of the third identification test (e.g., color, presence of bubbles) and determine whether the test results are positive or negative.
3. Explain what the results of the third identification test tell you about the traits of the unknown organism chosen in part A.
E. Identify your unknown organism based on your observed results and the “Decision Tree” web link.
Kirby-Bauer Diffusion Test
F. Describe how the Kirby-Bauer diffusion test in your lab manual determines whether a particular antibiotic can be used to treat an infection.
1. Describe whether the observed zone of inhibition measurement results from your Kirby-Bauer diffusion test indicate that the organism identified in part E is resistant, susceptible, or intermediately susceptible to each antibiotic tested, based on the provided “Data Table” from your lab manual.
2. Select the best antibiotic for treatment of the identified organism from part E based on the observed results from part F1.
G. Acknowledge sources, using in-text citations and references, for content that is quoted, paraphrased, or summarized.
H. Demonstrate professional communication in the content and presentation of your submission.
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