Designing of biosensor for detection of the gut micro bacteria.
Title
Introduction: Various type of bacteria are present in the gut. Some are beneficial for us and some causes disease. The early detection of bacteria helping understanding the pathogenesis and treatment of diseases even Colorectal cancer is because of the bacteria. So early detection it can be done by the double up and up of suitable markers.
How we can the design of suitable markers?
Designing of experiment:
1. Selection of bacteria: So different types to bacteria is present but we will select the bacteria which causes Colorectal cancer or be enhance the Colorectal cancer and will isolate the bacteria and we see how many different types of metabolites the bacteria is going to release.
In the laboratory condition as well as in the gut, so we will isolate or collect the various type of metabolites release by the bacteria from the faeces. Apart from this we will also isolate the metabolites release by this bacteria in the laboratory condition. After releasing this, these metabolites are further undergo this chemical reaction to produce a colour. So different in there many metabolites but we will select only that metabolites which is having a very high concentration release in very high quantity and which show increase in the concentration with respect to the progression of the disease that means if a person is suffering from Colorectal cancer stage-3rd must have higher concentration of this metabolite as compare to the stage-2nd. So that when this metabolite is treated with a chemical to produce colour by the intensity of the colour we can identify which stage of the cancer it is.
Expected result: So by this way we can design a suitable biomarkers for the early diagnosis of the disease.
