Daphnia Hypoxia RubricThe purpose of this lab report is to assess the impact of

Daphnia Hypoxia RubricThe purpose of this lab report is to assess the impact of hypoxic conditions on Daphnia. You willuse multiple primary sources and data from your experiments to substantiate the claim thatDaphnia produce hemoglobin when exposed to hypoxic environments.1. ABSTRACT (2) An overview of the findings from the paper. Begin with the major question being addressed,lead to its scientific relevance, followed by major findings, and any follow-up research thatshould be done.2. INTRODUCTION (8)Be sure to include information from your primary sources in your intro to support your claims!!!Context (4) Demonstrate a clear understanding of the big picture; why is this question important/interesting in the field of biology? Provide at least one explanation of why others would find the topic interesting and somerelevant context for the research question(s). Potential details to include (not an exhaustive list):o What is Hypoxia?o What is phenotypic plasticity?o Hif-1A, HB geneso Expectations under different conditions?Accuracy and relevance (2) Content knowledge is accurate, relevant and provides appropriate background for readerincluding defining critical terms. Background information is specific to content. Citations are relevant and INCLUDED!!! If you have no citations in your intro, your intro is a 0Hypothesis (2) Hypotheses are clearly stated, testable and consider reasonable alternative explanations At least one relevant, testable hypothesis is clearly stated. The hypothesis may be compared with a “null” alternative which is usually the absence of theexpected result. Details to include:o Density dependent effects of HIF1A/Hemoglobin expression?o Color change severity3. MATERIALS & METHODS (5)Procedure, Controls & Replication Procedure is clearly explained. Details to include:o Observation methodo Starting culture of daphnia
o Selection method (did you enforce a bias in which daphnia you selected for the falcontubes?)o Second observationso Analysis RNA Extraction cDNA conversion PCR of HIF1A, Hemoglobin, GAPDH, and ACTIN4. RESULTS (10)Results Text (5) Data are comprehensive, accurate and relevant. Details to include:o Observation of significant pigment intensity shiftso General trends in results from your primary sources This section should *NOT* juts repeat information contained in figures or tablesResults Figures/Tables (5) Data are summarized in a logical format with appropriate table or graph types. Properly labelthe data including units. Figures and graphs can include (at least 1):o Daphnia color change before and after for control and experimental groups (includingstatistics)o Daphnia behavior before and after for control and experimental groups (includingstatistics)o Daphnia survivability before and after for control and experimental groups (includingstatistics) Labeled PCR gel image from experiment Graph axes are appropriately labeled and scaled with informative and complete captions. The figure’s meaning is clear without requiring the reader to refer to the text. Details to include:o Descriptive figure title for all figures.o Figure caption with appropriate detail.o Labels on gel lanes, bands, etc.5. DISCUSSION (20)Conclusions based on the data selected (8) Conclusions are clearly and logically drawn from data provided. Relate back to source material (aka more citations!!!!) A logical chain of reasoning from hypothesis then to data and finally to conclusions is clearly andconvincingly explained.o Make sure to clearly define conclusions from all experiments. Tie it all together. Conclusions take limitations of the data into account.Alternative explanations / Limitations of data (5) Alternative explanations include possible hypothetical outcomes that may support conclusionsdrawn. Alternative explanations may be modifications of the original hypothesis and presentcorresponding conclusions. Conclusions take limitations into account.
 Details to include:o Use specific details on alternative explanation and limitations of the data, just sayinghuman error is inappropriate. Think critically about what may have happened in our experiments.Significance of research (7) Paper gives a clear indication of the significance of the research and its future directions Suggests work that would fill knowledge gaps and move the field forward.o This should be a well-reasoned future experiment, not just “we could dodifferent water levels!”o Include what you expect to see in your proposed experimento Include requirements for and limitations of your proposed experiment6. WRITING QUALITY AND CITATIONS (5) Spelling and use of grammar are mostly correct. Word usage makes reader’s understanding easier (aka no unnecessary jargon). A clear organizational strategy is present with a logical progression of ideas. The information ispresented in an easy to read format. YOU MUST INCLUDE AT A MINIMUM 3 PRIMARY AND 1 SECONDARY CITATION. FAILURE TOINCLUDE CITATIONS RESULTS IN A 0 FOR THE PAPER